What is a dilution?

A dilution is a reduction in the concentration of a solution. This will include a stock solution (solution to be reduced) and a Diluent (reducing agent eg distilled water).

To calculate the new concentration of a diluted solution one must KNOW

– the Concentration of the Original or Stock solution { C1 },

– the Volume of the Stock solution{ V1 } ,

– the Volume of the Final solution {V2}.

First have to CALCULATE the V1 since the C1 and V2 are usually known

For example prepare 5ml of a 1:5 dilution from a stock of 10mg/ml

V1 = Volume of Final solution X Dilution factor

= 5ML X 1/5

=1ML OF STOCK SOL. To be used to make dilution.

Since you KNOW the Concentration of the Original or Stock solution { C1 }, the Volume of the Stock solution{ V1 } and the Volume of the Final solution {V2}.

This formula is used C1 V1 = C2 V2

C2 = C1 V1 = 10mg/ml x 1ml = 2mg/ml

V2 5ml

Therefore the concentration of 5ml of 1:5 dilution is 2mg/ml..