# Dilutionconcentration Formula

What  is a  dilution?

A dilution is a reduction in the concentration of a solution. This will include a stock solution (solution to be reduced) and a Diluent (reducing agent eg distilled water).

To calculate the new concentration of a diluted solution one  must KNOW

–  the Concentration of the  Original or Stock solution { C1 },

–  the Volume of the Stock solution{ V1 }  ,

–  the Volume of the Final solution {V2}.

First have to CALCULATE the V1     since the C1 and  V2  are usually known

For example prepare 5ml of a 1:5 dilution from a stock of  10mg/ml

V1  = Volume of Final solution  X  Dilution factor

=  5ML                                  X  1/5

=1ML OF STOCK SOL. To be used to make dilution.

Since  you KNOW the Concentration of the  Original or Stock solution { C1 }, the Volume of the Stock solution{ V1 }  and the Volume of the Final solution {V2}.

This formula is used C1  V1 =  C2  V2

C2  =  C1  V1          = 10mg/ml  x 1ml  =      2mg/ml

V2                         5ml

Therefore the concentration of 5ml  of 1:5  dilution is 2mg/ml..